Construction and biological properties of chitosan/antimiR-138 on microporous titanium oxide surface
WU Kai-min
LIU Meng-yuan
SONG Shen-lin
FANG Ke-da
ZHANG Li
MENG Fan-hui
SHI Meng-qi
ZHANG Yu-mei
LIU Min
Abstract：AIM:To fabricate CS/antimiR-138 modified microporous surface on Ti implants.METHODS:The CS/antimiR-138 complexes were fabricated, and then loaded onto the MAO Ti surface by silane glutaraldehyde coupling.The MAO titanium surface was respectively loaded by CS/antimiR-138, CS/antimiR-control,4 silane glutaraldehyde coupling agent (MAO-SGC) and the naked MAO(MAO)(n=5).Water contact angle was measured.SEM was used to observe the surface morphology.Bone marrow mesenchymal stem cells(MSCs)were cultured on the specimens.The cell viability was examined by CCK-8 assay.The expressions of miR-138 and osteogenesis-related genes of the cells were evaluated by real-time PCR.RESULTS:The water contact angles of MAO, MAO-SGC and MAO-CS/antimiR-138 groups increased in turn significantly.The cell viability was not significantly different among the groups(P>0.05).MAO-CS/antimiR-138 group exhibited lower expression of miR-138(P<0.05) and higher expression of the osteogenesis-related genes than the other 3 groups(P<0.05).CONCLUSION:Covalent bonding of CS/antimiR-138 onto MAO titanium surface can improve osteogenic differentiation of MSCs without cytotoxicity.
Keywords：titaniumchitosanimplantmiRNA
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 442-448 )