The effects of TGF-β1 on the osteogenic differentiation of periodontal ligament stem cells
DING Xin-xin
LI Shu-hui
MA Yu
YIN Yan-jiao
GE Qiao-cheng
XIE Chao
WANG Xue
WU Pei-ling
Abstract：AIM:To evaluate the effects of TGF-β1 on the osteogenesis of human periodontal ligament stem cells (hPDLSCs).METHODS:hPDLSCs were in vitro cultured with TGF-β1 at 0 ng/mL (control group), 1 ng/mL and 10 ng/mL respectively.CCK8 assay, alkaline phosphatase(ALP) activity assay, alizarin red staining and real time quantitative PCR were conducted for the evaluation of the osteogenesis of hPDLSCs.RESULTS:TGF-β1 promoted cell proliferation, and 10 ng/mL TGF-β1 was the most effective.ALP in the experimental groups was higher than in the control group, in 10 ng/mL group was higher than in 1 ng/mL group (P<0.05).Mineralized nodule formation in the experimental groups was significantly more than in the control group, and in 10 ng/mL group was more than in 1 ng/mL group.The mRNA expression of osteopontin(OPN), osteocalcin(OCN), Runt-related transcription factor 2(RUNX-2) and Cementum Attachment Protein(CAP) were significantly increased in 10 ng/mL TGF-β1 group(P<0.05).CONCLUSION:TGF-β1 is a potent regulator of osteogenesis differentiation of human periodonal ligament cells.
Keywords：periodontal ligament stem cellsTGF-β1osteogenesis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 437-441 )