Adhesion and proliferation of human dental pulp cells on an injectable PLGA microsphere scaffold
WANG Guan-hua
SONG Fang
SHI Xu-dong
ZOU Hui-ru
Abstract：AIM:To observe the adhesion and proliferation of human dental pulp cells (HDPCs) at different density on an injectable polylactic-co-glycolic acid (PLGA) microsphere scaffold.METHODS:HDPCs of fourth passage were seeded onto PLGA microsphere scaffolds at 2×105, 1×105 and 5×104cells/mL respectively (Group A, B and C).Cell adhesion and proliferation were observed under inverted microscope.Double stranded deoxyribonucleic acid (dsDNA) content and alkaline phosphatase (ALP) activity were examined.After 8 weeks of culture, the ultrastructure was observed under SEM.RESULTS:Adhesion of HDPCs on PLGA microspheres was observed, the cells proliferated well and fused together.After 1 week, there was no significant differences in the cells dsDNA content and ALP activity among 3 groups.After 2 weeks, Group A and B showed significantly higher dsDNA content than Group C.Group B showed the highest ALP specific activity.After 8 weeks of culture, SEM showed that cells and scaffolds were completely fused together.There were matrix deposition and hollow sinking on the scaffolds.CONLUSTION:1×105cells/mL of HDPCs seeded onto PLGA microsphere scaffolds may produce better results of dsDNA content and ALP activity.
Keywords：human dental pulp cells(HDPCs)polylactic-co-glycolic acid microspherespulp regeneration
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 373-377,427 )