The role of microRNA-214 in the differentiation and mineralization of cementoblasts
HUI Hong-bin
DING Yu
FU Jian-jun
QU Xiao-bing
Abstract：AIM: To study the role of microRNA-214 in the differentiation and mineralization of cementoblasts.METHODS: Silencing lentiviral vector of microRNA-214 was constructed and then transfected into mouse cementoblasts OCCM-30 cell line.The transfection efficiency and silencing effects of the construct were observed by fluorescence microscope.The mineralization ability of cementoblasts was examined by Alizarin Red and Von kossa staining.The differentiation ability of cementoblasts was tested by Real-time PCR.RESULTS: The fluorescence microscope showed that the silencing lentiviral vector of microRNA-214 could be successfully transfected into cementoblasts.Differentiation of cementoblasts was demonstrated by the presence of the differentiation markers.The Alizarin Red and Von kossa staining demonstrated that the microRNA-214 silencing construct could boost the differentiation and minerlization ability of cementoblasts.CONCLUSION: Silencing of microRNA-214 can promote the differentiation and mineralization ability of cementoblasts.
Keywords：microRNA-214lentiviral vectorcementoblastsdifferentiation and mineralization
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 315-319,314 )