Differential expression of target genes and gene function of miRNAs in human stem cells and non-stem cells from apical papilla
LIN Shi-han
HU Xue-gang
LYU Hong-bing
Abstract：AIM: To study the differential expression of target genes and gene function of miRNAs in human stem cells and non-stem cells from apical papilla (SCAPs and NSCPs).METHODS: SCAPs were isolated by immune-magnetic binding specific STRO-1 antibody separation system.Osteogenic and adipogenic differentiation of SCAPs were tested by alizarin red staining(ARS) and Oil Red O staining.Differential miRNAs of SCAPs and NSCPs were screened by the Next generation sequencing.Target genes and their possible roles were predicted using biological information.RESULTS: The results revealed that 10 miRNAs (hsa-miR-4532, hsa-miR-6131, hsa-miR-4284, hsa-miR-3182, hsa-miR-1273e, hsa-miR-7-1-3p, hsa-miR-31-3p, hsa-miR-3654, hsa-miR-495-3p, hsa-miR-382-3p) were downregulated while 10 miRNAs (hsa-miR-4508, hsa-miR-1247-5p, hsa-miR-6819-3p, hsa-miR-2116-3p, hsa-miR-490-5p, hsa-miR-335-5p, hsa-miR-1914-5p, hsa-miR-1249, hsa-miR-3180-3p, hsa-miR-668-3p) were upregulated in SCAPs compared with those of NSCPs (P<0.05).26 target genes which mainly involved in the self-renewal capability, cell differentiation and migration were found.CONCLUSION: The target genes and gene function regulated to specific miRNAs of SCAPs are closely related to the cell stemness.
Keywords：stem cells from the apical papilla (SCAPs)miRNAstargets genesgene functionstemness
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 307-314 )