The correlation between chronic periodontitis with halitosis and TNF-α, IL-6, IL-10 and HMGB in peripheral blood

JIANG Yang; HE Quan-min; LIU Qing-meng

doi:10.15956/j.cnki.chin.j.conserv.dent.2017.05.004

Abstract：AIM: To assess the levels of TNF-α,IL-6,IL-10 and HMGB-1 in peripheral blood from the patients with chronic periodontitis,and the correlation with halitosis and dental plaque index(PLI).METHODS: 20 patients with severe chronic periodontitis and oral malodor(group Ⅰ),12 with simple oral malodor(groupⅡ),12 with mild chronic periodontitis without halitosis(group Ⅲ) and 8 healthy subjects without periodontitis and halitosis (control group) were included.The levels of TNF-α,IL-6,IL-10 and HMGB-1 in peripheral blood were examined with double antibody sandwich ELISA.The organoleptic rating (OR) was evaluated by organoleptic method and PLI was measured.The data was analyzed by SPSS 17.0.RESULTS: The levels of TNF-α,IL-6 and HMGB-1in peripheral blood of groupⅠand Ⅲ were higher than those of control group (P<0.05).The levels of TNF-α,IL-6,IL-10 and HMGB-1 of groupⅠ were higher than those of groupⅡ(P<0.05).The HMGB-1s levels in groupⅠand Ⅱwere higher than that those in group Ⅲ(P<0.05).The PLI level of groupⅠwas higher than that of group Ⅱ(P<0.05).In groupⅡ the OR level was higher than that in group Ⅲ(P<0.05).In group Ⅲ,OR was positively correlated with PLI (P<0.05).In group Ⅰ,OR and PLI were positively correlated with TNF-α,IL-6 and HMGB-1(P<0.05).In group Ⅱ PLI was positively correlated with HMGB-1 (P<0.05).OR and PLI levels in group Ⅲ were positively correlated with HMGB-1 (P<0.05).CONCLUSION: Over-expression and release of TNF-α,IL-6,HMGB-1,and especially HMGB-1,may contribute to chronic periodontitis and stubborn bad breath.