Resveratrol pretreatment promotes in vitro proliferation of periodontal ligament stem cells derived from aged donors
Abstract：AIM: To investigate whether resveratrol(AV) can promote the proliferation and colony-forming of periodontal ligament stem cells (PDLSCs) derived from aged donors.METHODS: The PDLSCs were acquired from the periodontal ligament tissues of middle-aged and elderly subjects by enzymolysis and cultured in vitro.the PDLSCs were not pretreated(control group) or pretreated by AV (AV group) or nicotinamide(NT group).Cell proliferation was determined by MTT assay and colony formation was detected by crystal violet staining.PCR and Western blotting were performed to determine the relative expressions of SIRT1 and HIF1-α.RESULTS: AV pretreatment enhanced the capacities of proliferation and colony formation of PDLSCs derived from aged donors (P<0.05),while NT impaired the proliferation and colony formation of the PDLSCs (P<0.05).The expression of SIRT1 was promoted and the expression of P53 was down-regulated by AV pretreatment (P<0.05).CONCLUSION: Resveratrol can reverse the proliferation and colony formation of PDLSCs derived from aged donors via the activation of SIRT1.
Keywords：periodontal ligament stem cellsresveratrolnicotinamidesenescenceSIRT1
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 254-260 )