Study on the oral microbial diversity differences between Han and Tibetan people with high caries risk in Qinghai

LI Zhi-yan; ZHAO Chong; LIU Jing; ZHANG Xin; ZHU De-rui; CHEN Yun

doi:10.15956/j.cnki.chin.j.conserv.dent.2017.05.002

Abstract：AIM: To study the difference of the diversity and composition of oral microorganism between Han and Tibetan people with high caries risk in Qinghai.METHOD: 10 Han chinese and 10 Tibetans with high caries risk were selected from outpatients.High-throughput sequencing method was performed by using an Illumina miseq sequencing platform for studying bacterial 16S rRNA gene V3-V4 region,then the microbial community structure and diversity were analyzed with the Mothur software.RESULTS: Based on 97% similarity clustering,the numbers of Operational Taxonomic Units (OTUs) in Han and Tibetan groups were 51 and 30 respectively.Oral microorganisms in the Han group were classified into 4 phyla,7 classes and 36 genera,and those in the Tibetan group were classified into 2 phyla,4 classes and 27 genera.The 12 dominant species in the Han group included Pseudomonas(32.52%),Enterobacteriaceae unclassified(29.91%),Leuconostoc (10.56%),Mesenteroides(4.71%),Lactococcus(3.71%) and Streptococcus(3.04%),et al,4 dominant species in the Tibetan group were Bacteria TM7-3(55.86%),Bacteria SR1(20.36%),Xanthomonadaceae unclassified(17.38%) and Pseudomonas (2.46%).CONCLUSION: The oral microbial diversity and abundance in the Han group are significantly higher than those in the Tibetan group,there is a significant difference in the dominant oral microorganisms between the Han and Tibetan group in Qinghai region.