The effects of static mechanical strain on the osteogenetic differentiation potential and cytoskeletal reorganization of human periodontal ligament stem cells derived from chronic periodontitis tissue
Abstract：AIM:To investigate the effects of static mechanical strain(SMS) on the osteogenic differentiation potential and cytoskeletal reorganization of human periodontal ligament stem cells derived from chronic periodontitis tissue(PPDLSCs).METHODS:PPDLSCs were primarily cultured and identified.After being subjected to cyclic SMS with physiological loading of 12% at a frequency of 0.1 Hz for 12 h using a FX-4000T system,mineralization of PPDLSCs was examined by ALP staining,alizarin staining and ALP activity test.Cytoskeletal arrangement was observed by immunofluorescence staining and confocal microscopy.Protein expression was evaluated by Western blot analysis.RESULTS:SMS decreased the density of mineralized nodules and suppressed the cytoskeletal rearrangement of PPDLSCs,down-regulated ALP,RUNX2,ROCK1,cofilin and Rho-A protein expression,increased p-cofilin and Rho-GDI protein expression.CONCLUSION:SMS in the magnitude of 12% can suppress the osteogenic differentiation potential and cytoskeletal reorganization of PPDLSCs.
Keywords：periodontal ligament stem cells derived from periodontitis tissue (PPDLSCs)cytoskeletonosteogenic differentiationstatic mechanical strain (SMS)
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 183-188 )