Screen of the differentially expressed genes in early developing murine dental follicle tissue based on GO analysis with whole genome microarray
ZHANG Chun-guang
NIE Min-yuan
MENG Qing-yan
WANG Hong-yun
AN Yu
DONG Rui
DANG Ping
Abstract：AIM:To screen the differentially expressed genes in early developing murine dental follicle tissue by whole genome microarray based on GO analysis.METHODS:The murine dental follicle at E17 and PN2 stages were dissected by LMD6000 system (Laser Micro Dissected system,Leica).To ensure the purity and the integrity of RNA samples,quality check was adopted by Bioanalyzer 2100 System,Agilent.For control and test RNAs,the synthesis of target cRNA probes and hybridization were performed using Agilent's Low RNA Input Linear Amplification kit.The fragmented cRNA was hybridized with assembled Agilent's Mouse Oligo Microarray.All microarray data normalization and selection of fold-changed genes were performed using GeneSpringGX 7.3.Functional annotation of the genes and GO analysis werer performed according to Gene OntologyTM Consortium by GeneSpringGX 7.3.Gene classification was based on searches by BioCarta,GenMAPP.RESULTS:A group of genes which were highly expressed in dental follicle compared to dental papilla tissue at both stages were obtained.More than 10 genes related to common biological functions such as "extracellular matrix" were obtained.CONCLUSION:The combination of laser microdissection and Microarray / bioinformatics analysis is a valuable means to obtain a global view of gene expression during the murine root / periodontal tissue development process.
Keywords：laser microdissectiontooth germ developmentmicroarraydifferential expression of genes
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 123-128,163 )