Sensory nerve regulates the formation of enamel and dentin in the adult rat incisor
Abstract：AIM:To investigate the role of sensory nerve in the formation of enamel and dentin in incisor of rats.METHODS:48 adult SD rats were divided into 4 groups (n =12) randomly.The rats received unilateral sensory nerve section (IANx),unilateral sympathetic nerve section (SCGx),unilateral sensory nerve and sympathetic nerve section (IANx + SCGx),and sham operation,respectively.The rats were sacrificed at the end of 2 W after operation and HE staining was performed to observe the changes of incisors.10 SD rats (4 W of age) were randomly divided into IANx group and sham group (n =5).rDPSCs were cultured and identified from the 2 groups of rats 2 W afte operation.The colony-forming ability,proliferation rate and expression level of genes associated with enamel and dentin formation were compared between the 2 groups.RESULTS:The incisors of IANx and IANx + SCGx groups turned chalky 2 W after denervation.HE staining showed reduced enamel and dentin formation (P ＜ 0.05).The rDPSCs of IANx group showed lower clonogenic and proliferative potential (P ＜0.05) and expressed lower levels of Amelogenin,Enamelin,Dspp and Dmp1 mRNA than sham group (P ＜0.05).CONCLUSION:Sensory nerve supports the formation of enamel and dentin by regulating rDPSCs.
Keywords：sensory nerveinferior alveolar nerveenameldentinsympathetic nervedental pulp stem cells
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 75-81 )