Oct4 rgulates the self-renewal capacity of human periodontal ligament stem cells
Abstract：AIM:To investigate the roles of Octamer binding transcription factor-4 (Oct4) on the self-renewal capacity of human periodontal ligament stem cells (hPDLSCs) in vitro.METHODS:hPDLSCs were isolated from human healthy periodontal tissues.Oct4 expression in hPDLSCs was detected by Real-time PCR after osteogenic induction.Then the proliferation,colony formation,adipogenic and osteogenic differentiation were evaluated after sliencing Oct4 gene of hPDLSCs.Meanwhile,the adipogenic and osteogenic related genes were assessed by Real-time PCR.RESULTS:Oct4 expression of hPDLSCs was down-regulated after osteogenic induction.After silencing Oct4 gene expression the proliferation,colony formation ability of hPDLSCs were inhibited.The mineral nodule and lipid droplet formation of hPDLSCs were also decreased significantly.Furthermore,the gene expression of PPARγ and DSPP,ALP,OCN and BSP were notably reduced.CONCLUSION:Oct4 plays an important role in maintaining the stem cell characteristics of hPDLSCs.
Keywords：Oct4hPDLSCscell differentiationcell proliferation
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 61-67 )