Periodontal ligament stem cell pellet: a potential method for regenerating bone tissue
DONG Hong-yu
MA Shu-fang
NA Si-jia
Abstract：AIM:To investigate the osteogenesis potential of periodontal ligament stem cell (PDLSC) pellet (CP).METHODS:PDLSCs were in vitro cultured,identified and CP was constructed.CP was examined by HE and IHC staining,scanning electron microscope(SEM) and real-time PCR for the observation of relevant osteogenic gene expression.Cell sheet (CS) was used as the control.CS and CP were respectively mixed with ceramic bovine bone (CBB) and then transplanted into the back of nude mice for the observation of bone formation.RESULTS:CP showed a better elasticity and shape.CP contained much more extracellular matrix (ECM) than CS,and CP was positive for the expression of alkaline phosphatase (ALP),bone sialoprotein (BSP) and collagen Ⅰ (COL-Ⅰ).In addition,CP expressed higher levels of osteogenic genes than CS counterparts.In vivo study revealed that CP group showed lots of bone-like tissues in CBB,but CS group showed a lot of fiber-like tissues.CONCLUSION:PDLSCs CP may be a new strategy for regenerating bone tissue.
Keywords：periodontal ligament stem cellcell pelletbone regenerationtissue engineering
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1-6 )