17β-estradiol enhances periostin expression in BMSCs of ovariectimized rats
YANG Xuan-xuan
CAO Meng
YANG Zhen-hua
XUAN Shi-jie
YANG Yan
LI Zhong
DING Yin
Abstract：AIM:To investigate the effects of 17β-estradiol(17β-E2) on the expression of periostin in rBMSCs of ovariectimized rats. METHODS:Ovariectomy ( OVX) was performed to establish osteoporosis in 6-w SD rats. rBMSCs were separated and cultured from Sham, OVX, and OVX+17β-estradiol treated rats, and then cell surface markers were indentified by flow cytometry. After osteoblastic induction, osteoblastic differentiation was evalua-ted by ALP activity and alizarin red staining. The expression level of periostin, Runx2, OCN and BSP was determined by RT-PCR. RESULTS: ALP expression and mineralization nodules were evidently observed in sham group, and significantly decreased in OVX group. 17β-E2 treatment increased the ALP expression and mineralization nodule for-mation. RT-PCR results showed that the expression of periostin, Runx2, OCN and BSP decreased significantly in OVX rats. 17β-E2 treatment significantly increased the expression of periostin, Runx2, OCN and BSP (P<0. 05). CON-CLUSION:17β-estradiol can enhance the expression of Periostin in BMSCs of ovariectimized rats.
Keywords：17β-estradiolperiostinosteoblastic differentiationrBMSCs
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 703-707 )