SLURP1 regulates the osteoclastogenic differentiation of stem cells from human exfoliated deciduous teeth through α7 nAChR
JIANG Yang
WANG Lu-lu
ZHOU Zhi-fei
ZHANG Bai-ze
WANG Jun-hui
WANG Xiao-jing
Abstract：AIM:To investigate the role of α7 nAChR and SLURP1 in differentiation and maturation of oste-oclasts in the process of deciduous teeth physiological root resorption.METHODS:SHEDs from different stages of de-ciduous teeth physiological root resorption and DPSCs from permanant teeth were isolated,cultivated and purified by en-zyme digestion method and limiting dilution assay.The cells were identified by cellular morphology,growth pattern,os-teogenic induction,adipogenic differentiation and flow cytometry analysis.Gene and protein expression of α7 nAChR was detected by quantitative real-time PCR,immunofluorescence and semiquantitative optical density analysis.SLURP1 and RANKL/OPG gene expression was detected by quantitative real-time PCR.RESULTS:Gene and protein expres-sion of α7 nAChR in SHEDs from the middle and the final stage of physiological root resorption was higher than those from the stable stage and higher than DPSCs(P <0.05).SLURP1 expression was the highest in the middle stage,fol-lowed by the final stage,permanent teeth and the stable stage of deciduous teeth.(P <0.05).RANKL/OPG ratio showed the similar expression tendency to SLURP1 expression.CONCLUSION:SLURP1 may regulate the osteoclas-togenic differentiation of stem cells from human exfoliated deciduous teeth by binding to α7 nAChR.
Keywords：physiological root resorptionstem cells from human exfoliated deciduous teethalpha7 nicotinic acetylcholine receptorsSLURP1
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 517-524,541 )