The function and mechanism of PDLSCs in orthodontic tooth movement
HE Yi-de
MA Xiao-jie
ZHANG Li-qiang
LIU Wen-jia
Abstract：AIM :To investigate the role of periodontal ligament stem cells (PDLSCs)in orthodontic tooth movement (OTM)and the effects of Wnt/β-catenin signaling pathway on the osteogenic differentiation of PDLSCs. METHODS:24 SD rats were randomly divided into 4 groups(n =6),orthodontic appliance was added in the upper right first molar for 0,1,3,and 7 d respectively.The animals were sacrificed and the histological sections were taken. The morphological changes of periodontal tissues were observed by HE staining.TRAP staining was used to observe the number of osteoclasts and immunohistochemical staining was used to observe the expression of nestin and active-β-catenin in periodontal tissues.Human PDLSCs were cultured and randomly divided into 3 groups.Cells in experimental group were treated with 100 KPa static pressure for 12 h and the controls were respectively treated with 0 KPa static pressure and low oxygen condition for 12 h,respectively.Western blotting was used to test the expression of OPG, RANKL,ALP,Runx2,p-GSK3βand active-β-catenin in each group.RESULTS:With the extension of orthodontic strength time,the periodontal space in the pressure side of orthodontic tooth became narrow and the number of osteo-clasts in the pressure side was gradually increased.The expression of nestin and active-β-catenin in periodontal tissues was increased constantly with time(P <0.05).Western blotting analyses indicated that,in hPDLSCs treated with 100 KPa/12 h static pressure,p-GSK3βand active-β-catenin expression was increased (P <0.05),while ALP and Runx2 expression was decreased (P <0.05)and the ratio of RANKL/OPG was increased (P <0.05).CONCLU-SION:PDLSCs play an important role in the orthodontic tooth movement.Wnt/β-catenin signaling pathway is activa-ted under static pressure,hence inhibiting the osteogenic activity and promoting the osteoclastic activity of PDLSCs.
Keywords：orthodontic tooth movement (OTM)periodontal ligament stem cells (PDLSCs)static pressurecanonical Wnt pathway
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 411-417,438 )