Screening and verification of the target genes of miR-23a and miR-30a in human periodontal ligament cells
CHANG Wen-yue
YANG Qian-juan
GUO Xiao-rui
LU Qun
Abstract：AIM:To screen and verify the target genes of miR-23a and miR -30a in human periodontal ligament cells (PDLCs).METHODS:Bioinformatic approaches including miRanda,TargetScan and PicTar were used to predict the potential target genes of miR-23a and miR-30a in PDLCs.Following miR-23a and miR -30a ex-pression intervention by simulated inflammation microenvironment in vitro,the mRNA and protein expression of the predicted target genes were examined in order to screen and verify the target genes of miR -23a and miR -30a.RE-SULTS:CAMTA1,CNTTBIP,PPPAR,Runx2,STAT1,STAT5B and SATB1 were predicted as the putative target genes of miR-23a and miR-30a.Real-time PCR showed that miR-23a and miR-30a gene intervention respectively af-fected the mRNA expression of STAT5B and Runx2.Western blot showed that the protein expression of STAT5B and Runx2 was respectively influenced by miR-23a and miR-30a gene intervention.CONCLUTION:MiR-23a and miR-30a are involved in the regulation of PDLCs bioactivities in the inflammatory microenvironment.STAT5B and Runx2 are respectively the target genes of miR -23a and miR -30a.
Keywords：target genesinflammationmicroRNA
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 401-406,410 )