The effects of superparamagnetic iron oxide nanoparticles on the biological activity of human dental pulp stem cells in vitro and in vivo
MA Liang
BAI Yu
LI Ming-wei
SUN Xue-fei
WANG Sheng-chao
YU Qing
Abstract：AIM:To explore the effects of superparamagnetic iron oxide nanoparticles (SPIO)on the bio-logical activity of human dental pulp stem cells (hDPSCs)in vitro and in vivo.METHODS:hDPSCs were incubated in culture medium with SPIO at 0 μg/mL,25 μg/mL,50 μg/mL and 100 μg/mL respectively for 24 hours.The labe-ling efficiency,viability,proliferation,cell cycle and apoptosis of the cells were examined.The cells labeled by 25 μg/mL SPIO were cultured in osteogenic induction medium and the osteogenic differentiation potential was observed in vitro.hDPSCs and SPIO labled hDPSCs were respectively combined with scaffold and implanted subcutaneous into nude mice,the in vivo bone formation was observed by Van Gieson staining 8 weeks after implantation.RESULTS:25 μg/mL -50 μg/mL SPIO had no influence on the cell vitality but promoted cell proliferation in 1 d -3 d culture. However,100 μg/mL SPIO showed toxic effect on cell vitality and inhibited the proliferation of hDPSCs.For osteogen-ic differentiation,no significant difference was found between control group and 25 μg/mL SPIO group both in vitro and in vivo.CONCLUSION:25 μg/mL SPIO can effectively and safely label hDPSCs,promote cell proliferation and has no influence on osteogenic differentiation.
Keywords：superparamagnetic iron oxidehuman dental pulp stem cellsosteogenic differentiationstem cell tracking
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 387-395 )