The effects of radices scutellariae extract on the prevention and treatment of osteoporosis in rats
GUI Lin-yuan
LIU Shi-yu
QIU Xin-yu
DING Yin
JIN Yan
Abstract：AIM:To inviestigate the effects of radices scutellariae extract on the prevention and treatment of oste-oporosis in rats.METHODS:Osteoporosis models of 6 female SD rats were established by ovariectomy .Scutellariae extract was injected intraperitoneally 1 w after ovariectomy in prevention group(n =3),while the extract was given 2 m after ovariectomy in treatment group(n =3).Micro CT scanning of femur was conducted 6 and 12 w after scutellariae extract injection,bone mass and trabecular bone volume were measured.3 rats underwent sham operation were served as the controls.Bone marrow mesenchymal stem cells (BMMSCs)were cultured with or without scutellariae extract , the proliferation and osteogenesis differentiation of BMMSCs were examined.RESULTS:Micro-CT results showed that the bone mass and trabecular bone volume of scutellariae extract group were higher than those of the control group. MTT assay and clone formation assay showed Scutellariae extract promoted BMMSCs proliferation.ALP and Alizarin red staining indicated that scutellariae extract induced osteogenesis differentiation.CONCLUSION:Scutellariae extract have the preventive and treatment effects on osteoporosis,and it can promote the proliferation and osteogenesis differen-tiation of BMMSCs.
Keywords：scutellariae extractosteoporosispreventiontrreatmentbone marrow mesenchymal stem cellsproliferationdifferentiation
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 294-300 )