The effects of dentin non -collagenous proteins on the proliferation and mineralization of human stem cells from the apical papilla
MA Zhao-feng
LI Shi
WENG Xi-li
YE Tao
SUN Dong-liang
YU Shi-bin
Abstract：AIM:To investigate the effects of dentin non-collagenous proteins (DNCPs)on the proliferation and differentiation of human stem cells from the apical papilla (hSCAPs)in vitro.METHODS:hSCAPs were treated with 10 μg/mL of DNCPs,and the proliferation of the hSCAPs was detected by MTT assay.To investigate the differen-tiation of hSCAPs,alkaline phosphotase (ALP)activity was determined by enzyme kinetics methods,the mRNA ex-pression of ALP,OCN and COL I was detected by real-time PCR,and the mineralization was detected by Alizarin red staining assay.RESULTS:The proliferation activity was not statistically different differect between the DNCPs-trea-ted,and the control cells(P >0.05).After 7 and 14 days culture in DNCPs containing medium,the mRNA expres-sions of ALP,OCN and COL I in hSCAPs were significantly upregulated(P <0.05).ALP activity of dNCPs-treated cells was significantly promoted(P <0.05).After 3 weeks,the calcified nodules in DNCPs-treated cells were bigger and more that those in the controls(P <0.05).CONCLUSION:DNCPs can promote the mineralization ability of hSCAPs,while have no significant effect on the proliferation ability.
Keywords：dentin non-collagenous proteinshuman stem cells from the apical papillaproliferationmineralization
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 283-287 )