The effects of helicobacter pylori vacuolating cytotoxin on the proliferation and apoptosis of human periodontal ligament fibroblasts
MA ling
XU Dan
REN Ji-fang
Abstract：AIM:To observe the effects of helicobacte pylori vacuolating cytotoxin (HP VacA)on the pro-liferation and apoptosis of human periodontal ligament fibroblasts (hPDLFs).METHODS:hPDLFs were cultured in vitro with different concentrations (0.1,0.2,and 0.3 mg/mL)of HP VacA.Cell proliferation was examined by CCK-8 method,the morphological changes were observed under inverted microscope,the apoptosis of the cells was detec-ted by flow cytometry (FCM),and changes of cell ultrastructure was observed by transmission electron microscope. RESULTS:HP VacA showed a concentration-and time-dependent inhibition on hPDLFs proliferation and stimulation on apoptosis.After treatment by HP VacA the number of adherent hPDLFs decreased.The cell shape gradually changed from long fusiform into rounded and suspended.Apoptotic cells increased as detected by FCM.A large num-ber of vacuoles within the cell cytoplasm was observed by transmission electron microscope,in which cell chromosomes were focus on karyotheca as massive,concentrated and marginalized presenting classical features of cell apoptosis. CONCLUTION:HP VacA can inhibit the proliferation of periodontal ligament fibroblasts and cause cell apoptosis.
Keywords：helicobacter pylori(HP)vacuolating cytotoxin(VacA)human periodontal ligaments fibroblasts (hPDLFs)apoptosis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 272-276 )