The effects of PI3K inhibitor LY294002 on the proliferation and osteogenic differentiation of human dental pulp stem cells
HE Xin-yao
LI Dong-mei
LIU Bao-gang
WANG Xiao-li
HE Wen-xi
Abstract：AIM:To investigate the effects of LY294002,a PI3K inhibitor,on the proliferation and osteo-genic differentiation of human dental pup stem cells (hDPSCs)in vitro.METHODS:DPSCs of the 3rd passage were treated with different concentrations of LY294002 for 1,3,5 and 7 days respectively and the cells were subjected to CCK -8 assay.The cells were induced with osteogenesis induction medium with LY294002 of different concentrations for 2 weeks.The mineralized nodule formation of the cells was observed by alizarin red staining,the relative mRNA ex-pression level of ALP,BSP and OCN was examined by RT-PCR.RESULTS:LY294002 promoted the proliferation of hDPSCs as time went on.LY294002 inhibited the osteogenic differentiation of hDPSCs in a concentration dependent manner.LY294002 down-regulated the mRNA expression of ALP,BSP and OCN.CONCLUSION:LY294002 pro-motes the proliferation of hDPSCs,while inhibits the osteogenic differentiation of hDPSCs,which indicates that PI3K signaling pathway may participate in the proliferation and osteogenic differentiation of hDPSCs.
Keywords：phosphatidylinositol 3-kinase (PI3K)LY294002human dental pulp stem cell (hDPSCs)cell proliferationcell differentiation
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 267-271 )