Bactericidal effect of Er:YAG laser with different apical working width on Enterococcus faecalisin experimentally infected root canals
TIAN Tian-tian
XIANG Dou-dou
LI Fen
TIAN Yu
YU Qing
Abstract：AIM:To investigate the bactericidal effect of Er:YAG laser with different irradiation time and apical working width on enterococcus faecalis in root canals.METHODS:Root canals of 125 human teeth with single straight canals were inoculated with Enterococcus faecalis for 4 weeks,10 teeth were randomly selected for bacteria baseline data at different depths.The remaining 1 15 teeth were randomly divided into 5 groups and treated as follows:① mechanical instrumentation +Er:YAG laser +52.5 g/L NaClO;②mechanical instrumentation +52.5 g/L Na-ClO;③ Er:YAG laser +52.5 g/L NaClO(E0:30 s,E1:for 1 m,E2:2 m);④52.5 g/L NaClO treatment for 3 m (and ⑤ normal saline for 1 m).After treatment,the numbers of bacteria on the surface of root canal wall at the depth of 0-100,100-200 and 200-300μm were counted and the bacterial reduction were analyzed by one-way ANOVA. RESULTS:The bactericidal efficiencies of Er:YAG plus NaClO were higher than that of 52.5 g /L NaClO and nor-mal saline (P<0.05 ).On the surface and at 0 -100 μm depth,the bacterial reduction of different duration of Er:YAG laser treatment were similar (P>0.05).But at depth of 100-200 μm and 200-300 μm,the bactericidal efficiency of group E0 was lower than that of groups E1 and E2 (P<0.05),E1 vs E2(P>0.05).Treatment with Er:YAG laser after enlarging the root canals to#30/0.04 showed more than 99%reduction of bacteria in the deep lay-ers (100-200 μm and 200 -300 μm)of the dentinal tubules.Group #20/0.04 vs group #40/0.04(P>0.05). CONCLUSION:Er:YAG laser can enhance the bactericidal effect of sodium hypochlorite.Furthermore,Er:YAG la-ser can reduce the width of canal preparation while achieve the same sterilizing effect.
Keywords：Er:YAG laserbactericidal effectminimal invasive root canal treatmentapical working widthnterococcus faecalis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 222-227,249 )