Effects of ALPL gene knockdown on the apoptosis of dental pulp stem cells
CHEN Qi
WANG Hong
LIU Wen-jia
YANG Zhen-hua
ZHANG Li-qiang
YANG Qian-juan
JIN Fang
Abstract：AIM:To investigate the effects of ALPL gene knockdown on the apoptosis of human dental pulp stem cells (hDPSCs).METHODS:hDPSCs of the third passage were divided into an experimental group with ALPL gene knockeddown by lentivirus and a control group without gene knockdown.Western blot was performed to detect AL-PL protein expression of the cells.Apoptosis of the cells was determined by flow cytometry,and the expression of caspase-3 and caspase-8 was examined by Western Blot.RESULTS:Western blot and ALPL staining results indica-ted that the ALPL knockdown corresponding virus particles can effectively knock down the expression of ALPL gene in hDPSCs,leading to higher apoptosis of the cells,and higher expression of caspase-3 and caspase-8 (P<0.05 ). CONCLUSION:ALPL knockdown promotes the apoptosis of hDPSCs.
Keywords：human dental pulp stem cells (hDPSCs)apoptosisalkaline phosphatase(ALP)transfection
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 218-221 )