The effects of aspirin on the proliferation and differentiation of human dental pulp stem cells and the underlying mechanisms
SUN Lei
FAN Xiao-min
HE Wen-xi
ZHANG Ya-qing
Abstract：AIM:To investigate the effects of aspirin on the proliferation and differentiation of dental pulp stem cells (hDPSCs).METHODS:hDPSCs were cuture in mineralization induction medium and treated with different concentrations of aspirin for different periods respectively.The proliferation of hDPSCs was examined by MTT assay. The mineralization of the cells was observed by alizarin red staining after aspirin treatment for 14 days.MAPK expres-sion of the cells was detected by Western blot.RESULTS:Aspirin at 0.005 and 0.05 mmol/L promoted the prolife-reation of hDPSCs,at 0.5 mmol/L showed a decreased stimulating effect.5 mmol/L aspirin inhibited the cell prolife-reation.Aspirin at 0.5 and 0.05 mmol/L reduced the formation of mineralized nodules.0.5 mmol/L aspirin time-de-pendantly decreased JNK and p38 expression while it showed no significant influence on ERK1/2 expression.CON-CLUSION:Aspirin may inhibits odontogenic differentiation of hDPSCs through inhibiting p38 and JNK MAPK signa-ling pathway.
Keywords：Aspirinhuman dental pulp stem cellsmineralization differentiationproliferationMAPK pathway
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 213-217 )