The effects of strontium micro/nano-scale coating on the biological activity of BMMSCs
FU Qian
LI Yong-feng
CHENG Bing-kun
LIANG Jian-fei
QIN Dong-ze
XU Shan-shan
QI Ya-ping
NIU Qiang
KONG Liang
Abstract：AIM:To evaluate the effects of strontium micro/nano scale coating on the biological activity of BMMSCs.METHODS:Titanium plates were divided into 3 groups:polished surface group (n =24,group A), Hydrofluoric (HF)acid etched surface group (n=24,group B),and HF acid etched plus magnetron sputtered sur-face group (n=27,group C).The surface properties of the plates were observed by SEM.The elemental composition was assessed by an energy dispersive X-Ray spectroscopy detector (EDS).The hydrophilia was evaluated by contact angles analysis.Sr2+release was examined.BMMSCs were seeded onto the surface of the plates,early adhesion and morphology of BMMSCs were observed by confocal microscopy and SEM.Cell viability and ALP activity were detected by MTT assay and ALP activity detection kits respectively.RESULTS:Micro-scale surface structure was formed after HF etching or after HF acid etched plus magnetron sputtering.The contact angle of the plate surface of group B and C was smaller than that of group A.The strontium was loaded on the samlde surface and could be released in group C. The cells in group B and C showed higher adhesion and proliferation than those in group A.However,Group C showed the highest ALP activity among the 3 groups.CONCLUSION:The strontium-coated micro/nano scale surface can promote the proliferation and osteogenic differentiation of BMMSCs.
Keywords：surface modificationmagnetron sputteringstrontiummicro/nanoBMMSCs
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 208-212,217 )