The effects of cyclic hydrostatic pressure on the mechano-growth factor expression in periodontal ligamengt stem cells
ZOU De-hui
ZHAO Ying
ZHAO Yin-hua
CHEN Yong-jin
ZHANG Min
Abstract：AIM:To investigate the effects of periodic cyclic hydrostatic pressure on the expression of mech-ano-growth factor (MGF)in human periodontal ligament stem cell(hPDLSC)sheets.METHODS:hPDLSC sheets were prepared passage 3 hPDLSC sheets were treated by cyclic hydrostatic pressure at 0 KPa (control),0-90 KPa, 0-120 KPa and 0-150 KPa for 1 h/0.1 Hz,respectively.At different time points (0,12,24 and 36 h respective-ly),cell proliferation rate was measured by CCK-8,the mRNA expression of MGF,ALP and Col-Ⅰwas examined by real-time PCR .RESULTS:The pressure of 0 -120 KPa promoted cell proliferation (P<0.05 ).Hydrostatic pressure stimulation caused a quick increase of MGF mRNA at 12 h,MGF mRNA reached peak at 24 h and returned to normal control level at 36 h.0 -120 KPa group showed the strongest stimulating effect on MGF mRNA expression. Furthermore,it also increased ALP and Col-I expression (P<0.05).CONCLUSION:Cyclic hydrostatic pressure at 0-120 KPa /1 h may promote the expression of MGF and increase the proliferation and differentiation of hPDLSCs.
Keywords：human periodontal ligament stem cells(hPDLSCs)hydrostatic pressuremechano-growth factor(MGF)
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 191-196 )