In vivo study of osteogeneticablity of porous titanium modified by nanotubes

CHANG Bei; HAN Tian-xiao; LI Fu-ping; KOU Hong-chao; ZHANG Yu-mei

doi:10.15956/j.cnki.chin.j.conserv.dent.2016.01.005

Abstract：AIM:To explore the effects of porous titanium with nanotubes on bone formation in vivo. METHODS:Porous titanium were fabricated by vacuum diffusion bonding and the nanotubes were formed by anodic oxidation with the voltage of 20 V and 25 V respectively.The surface morphology of the samples was observed by SEM. 1 2 samples with nanotubes on the surface(experimental group)and 1 2 samples without nanotubes(control group)were implanted in the defects of femur condylus of 1 2 rabbits with sequential fluorescent labeling.The samples with sur-rounding bone tissue were harvested after 1 and 3 months respectively.The hard tissue sections were prepared and bone formation was examined by Micro -CT.RESULTS:Nanotubes with the diameters of 80 nm were fabricated by anodic oxidation with the voltage of 25 V on the surface of the porous titanium.Fluorescent labeling results showed that after 1 month,the experimental group exhibited higher osteogenic ability than the control group.Micro -CT examination showed that after 1 month,the bone volume /total volume (BV /TV)and trabecular number (Tb.N)of the experimen-tal group were higher than those of control group (P <0.05),while the trabecular space (Tb.Sp)of the experimental group was smaller (P <0.05 ).No difference was observed between the 2 groups (P >0.05 )after 3 months. CONCLUSION:Porous titanium with nanotubes may improve osteogenetic ability at early stage of bone healing and the integration of titanium with surrounding bone.