The influence of sucrose and xylitol on the interaction between Streptococcus sanguis and Streptococcus mutans

ZHAO Guang-jie; ZHAO Yan-xia; JIANG Xin-ya; LI Bei-lei; CHU Jin-pu; LIU Xue-jun

doi:10.15956/j.cnki.chin.j.conserv.dent.2016.01.002

Abstract：AIM:To investigate the influence of different concentrations of sucrose and xylitol on the pro-duction of hydrogen peroxide by Streptococcus sanguis (S.s)and the interaction between S.s and Streptococcus mutans (S.m).MTHODS:The production of H2 O2 by S.s was determined by o -Dianisidine dihydrochloride -horseradish peroxidase with different sucrose and xylitol concentrations.The interaction between S.s and S.m was observed by plat-ing method with carbohydrates in both aerobic and anaerobic environment.RESULTS:Under aerobic environment with different carbohydrates the total production of H2 O2 by S.s was as following:BHI +50 mmol/L sucrose >BHI +5 mmol/L sucrose >BHI >BHI +5 mmol/L xylitol >BHI +50 mmol/L xylitol(P <0.05).After H2 O2 concentration being adjusted to the absorbance of the bacteria suspession at 600 nm,the result was BHI >BHI +5 mmol/L sucrose >BHI +50 mmol/L sucrose >BHI +5 mmol/L xylitol >BHI +50 mmol/L xylitol(P <0.05).The inhibition was not ob-served when S.s and S.m were inoculated simultaneously.The H2 O2 production inhibition was the strongest when the bacteria were culture with 50 mmol/L sucrose and stronger in anaerobic environment when S.m was inoculated first. The inhibition was the strongest with 50 mmol/L sucrose and stronger in aerobic environment when S.s was inoculated first.CONCLUSION:The H2 O2 production by S.s is influenced by sucrose and xylitol concentrations .The interac-tion of S.s on S.m is influenced by inoculation time and sequences,different kind of carbohydrates and the oxygen con-centration.