Investigation of a novel PECT based drug delivery system of IPF loaded amphiphilic thermosensitive hydrogel
JIA Yu-rong
YIN Li
HUANG Li-xun
WANG Yong-lan
DONG An-jie
Abstract：AIM:To investigate the biosafety, anti-inflammatory effect and drug-release property of a no-vel PECT-based drug delivery system of ibuprofen( IPF) loaded amphiphilic thermosensitive hydrogel. METHODS:IPF/PECT nanoparticles ( IPF/PECT NPs) were manufactured by nanoprecipitation, then the particles were freeze-dried and dissolved into double distilled water to become a liquid solution. The morphology of the particles was ob-served by TEM, the anti-inflammatory effect of IPF/PECT nanoparticles was examined by MTT and ELISA with a hu-man gingival fibroblasts( HGFs) based inflammation model. RESULTS:The particles were uniform-sized and no ag-gregation was observed. After loading different concentrations (0, 200, 400, 800, 1,200, 1,600 and 2,000 μg/mL respectively) of IPF/PECT, the means of OD value of HGFs were 0. 29 ± 0. 01, 0. 28 ± 0. 01, 0. 29 ± 0. 02, 0. 29 ± 0.03,0.29±0.03,0.29±0.03,and0.28±0.02respectively(betweeneach2groups,P﹥0.05). Atevery point-in-time, the IPF/PECT group showed lower PGE2 level than IPF group(P<0. 05). The total release process could last for 32 days, with a cumulative release amount of 85%. CONCLUSION:The novel PECT-based drug de-livery system is biocompatable with slow-releasing property and anti-inflammatory effect.
Keywords：ibuprofen( IPF)hydrogelamphiphilicsustained-releasedrug-loaded
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 587-591 )