Bioactive glass 58 S/silk fibroin membrane premotes proliferation and differentiation of human dental pulp stem cells
LYU Xiao-shuai
LI Zheng-mao
YANG Xue-chao
Abstract：AIM:To investigate the effect of bioactive glass 58S/silk fibroin (BG58S/SF) membrane on the proliferation and differentiation of human dental pulp stem cells ( hDPSCs) . METHODS:hDPSCs were seeded on pure SF membrane and BG58S/SF with BG58S at 1 mg/mL and 5 mg/mL respectively after the materials were incubated in cell culture medium for 24 hours. Cell proliferation was assessed by CCK-8 kit after 4, 7, 14 and 21 d culture re-spectively. The adhesion of hDPSCs on the material surface was evaluated by SEM and cytoskeleton staining;ALP ac-tivity was measured by ALP kit and the expression of odontoblastic differentiation -related genes was measured by RT-PCR. One-way ANOVA and paired test were used for statistical analysis . RESULTS: hDPSCs proliferated well on the surface of the membranes throughout the culture period. ALP activity was enhanced in all the 3 groups from day 7, BG58S/SF membrane groups showed higher ALP activity than SF group (P<0. 05). BG58S/SF groups showed higher up-regulation of odontoblastic differentiation-associated genes than to SF group (P<0. 05). ALP activity and orodontoblastic differentiation-associated gene expression level in 5 mg/mL BG58S/SF group was higher than that in 1 mg/mL group (P<0. 05). CONCLUSION:BG58S/SF membrane may promote the proliferation and odontoblastic differentiation of hDPSCs.
Keywords：bioactive glasshuman dental pulp stem cellssilk fibrointissue engineering
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 577-583 )