The construction and preliminary study of matrix extracellular phosphoglycoprotein gene promoter
GONG Chun-mei
ZHANG Juan-juan
XIA Tian-yong
SUN Yan
Abstract：AIM: To study the molecular mechanisms of MEPE gene promoter expression in preosteoblasts in mouse. METHODS:MEPE gene promoter of mouse preosteoblasts was analysed by software( fruitfly. org/ seq_tools/promoter. html) , five candidate sequences were obtained. the primers for the construction of different lengths of MEPE gene promoters were designed; dual luciferase reporter assay was applied to analysis the effects for transcription activity of MEPE gene promoters with different lengths. The effects of BMP2 on the transcription activity of MEPE gene promoters in preosteoblasts were observed. RESULTS:MEPE gene promoters with the lengths of P-78~+66, P-140~ +66, P-300~ +66, P-500~ +66 and P-1081~ +66 were obtained. Double luciferase genetic testing report system analysis showed that the transcription activity of different lengths MEPE gene promoters was differ-ent(P<0. 05) . The transcription activity of MEPE gene promoter was significantly upregulated in the area of -140~-500 after BMP2 treatment. CONCLUSION:The location of MEPE gene promoter in mouse preosteoblasts was re-constructed. BMP2 may regulate the MEPE gene promoter at -140~ -500 area.
Keywords：MEPEgene cloneBMP2dual luciferase reporter assay
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 519-523 )