The expression of Jun family proteins in enamel development and the effect on amelogenin
XU Chang
Wei Ya-hong
WANG Yu-min
ZHANG Li
HAN Xiao-qian
HAN Ting-ting
GAO Yu-guang
Abstract：AIM:To investigate the role of Jun family proteins in enamel development of mouse. METH-ODS:Immunohistochemical staining was applied to detect the expression of JunB, c-Jun and JunD in mouse tooth germ ameloblasts. RT-PCR was employed to evaluate the effect on the mRNA expression of amelogenin after transfec-tion of the ameloblasts by JunB, C-Jun and JunD at 0. 5 μg and 2. 0 μg respectively. RESULTS: At the secretion stage, JunB was not expressed and JunD was weakly expressed in ameloblasts nuclei, but c-Jun was obviously ex-pressed. At the transition stage, JunB and JunD expression were increased and JunD was earlier than JunB. c-Jun continuously exhibited strong expression. At the maturation stage, all of the proteins were strongly expressed. Transfec-tion results showed that high dose of JunB inhibited the expression of amelogenin. Low dose of c-Jun evidently en-hanced the expression of amelogenin JunD had no significant effect on amelogenin expression. CONCLUTION:c-Jun may up-regulate the expression of amelogenin at the early stage of enamel development. JunB may suppress the secre-tion of amelogenin and promote the maturation of enamel at the late stage of enamel development. JunD has no significant effect on amelogenin.
Keywords：Jun family proteinsamelogeninimmunohistochemistryRT-PCR
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 515-518,528 )