Effects of hydrofluoric acid-treated titanium micro/nano surface on the biological activity of bone marrow mesenchymal stem cells
SHEN Ming-ming
LI Yan-rong
QI Ya-ping
FU Qian
QIN Dong-ze
LIANG Jian-fei
ZHAO Yun-zhuan
Abstract：AIM:To evaluate the effects of hydrofluoric ( HF) acid-treated titanium surfaces on the biologi-cal activity of bone marrow mesenchymal stem cells ( BMMSCs ) . METHODS: Titanium plates were divided into 3 groups, the suface of the samples was polished in group A, etched by 12. 5 g/L HF acid for 1 min group B and etched by 12. 5 g/L HF acid for 15 min Group C. The surface of the sample was observed by scanning electron micros-copy ( SEM ) . The suxface chemical composition was assessed by energy dispersive X-Ray spectroscopy detector ( EDS) . Contact angles were detected by drop method . BMMSCs adhesion and morphology were examined by confocal microscopy and SEM. Cell viability and ALP activity were assessed by MTT assay and ALP detection kit. RESULTS:Micro/nano-scale surface structures were formed after HF etching. The sample surfaces of group B and C were loaded with fluorine. Contact angle of etching groups was significantly smaller than that of polishing group. HF-treated titani-um surfaces significantly promoted adhesion, proliferation and ALP activity of BMMSCs. Furthermore, the effects of group C was greater than those of group B. CONCLUSION:HF acid-etch may forme different size of micro/nano-scale structures and load with fluorineon on titanium surface;can improve the biological activity of BMMSCs.
Keywords：HFacid etchingmicro/nanosurface modificationbiological activity
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 472-476,500 )