MiR-21 regulates osteogenesis of human marrow-derived mesenchymal stem cells
YANG Nan
ZHOU Wei
WANG Guang
DING Yin
JIN Yan
Abstract：AIM:To investigate the expression and the role of miR-21 in osteogenesis of human marrow-derived mesenchymal stem cells ( hBMMSCs) . METHODS:The expression level of miR-21 after transfection of syn-thetic per-miR-21 was confirmed by real time RT-PCR. After 2 days of transfection, hBMMSCs were induced to os-teoblast differentiation. ALP and Alizarin red S staining were used to determine osteogenic ability. Real time RT-PCR and western blot were used to analyze the estrogenic marker gene expression. hBMMSCs transfected with pre-miR-21, anti-miR-21 or miR control were loaded on hydroxyapatite-tricalcium phosphate scaffold and implanted s. c. in NOD/SCID mice. 8 weeks after transplantation HE staining and Masson 's trichrome staining were used to observe the new bone formation. RESULTS:miR-21 was increased during osteogenic differentiation of hBMMSCs. Gain-loss-functional analysis showed that up-regulation of miR-21 promoted osteogenic differentiation of hBMMSCs in vitro and in vivo. On the contrary, silencing of miR-21 inhibited the osteogenic differentiation of hBMMSCs in vitro and in vivo. CONCLU-SION:miR-21 can promote osteogenic differentiation of hBMMSCs in vitro and enhance ectopic bone formation in vivo.
Keywords：miR-21human bone marrow-derived mesenchymal stem cells (hBMMSC)osteogenic differentiation
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 465-471 )