Effects of MMP-3 on the reparative dentin formation in rats after dental pulp injury
Abstract：AIM: To study the role of MMP-3 on the reparative dentin formation after pulp injury. METHODS:48 Wistar rats were divided into 3 groups(n=16). A cavity on the occlusal surface of maxillary first mo-lars on left side was prepared in each rat, PBS, calcium hydroxide, and MMP-3 was applied on the exposed pulp in the rats of the 3 groups respectively. On day 3, 7, 14 and 28 after operation, the rats were sacrificed. The tooth were stained with HE and immunohistochemistry. DMP-1 expression was observed. RESULTS:In MMP-3 group, DMP-1 was weakly expressed on d 3, positively expressed on d 7 and strongly expressed on d 14, but its expression decreased on d 28 in odontoblasts. A large number of scattered osteoid dentin was observed under the exposed pulp on d 7. Con-tinuous calcified bridge was observed under the exposed pulp on d 14 . A thick layer of reparative dentin was observed under the exposed pulp, MMP-3 group showed stronger DMP-1exoression(P<0. 05) and more reparative dentin than PBS group at each time point, and than calcium hydroxide group on d 3 and d 5. CONCLUSION:MMP-3 may pro-mote the repair of dental pulp after injury.
Keywords：matrix metalloproteinase 3(MMP-3)dental pulpwound healingodontoblas
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 453-458 )