Effects of phenytoin on the proliferation and tumor necrosis factor-αexpression in LPS-treated human periodontal ligament fibroblasts
YU Fang-fang
YE Xin
CHEN Dong
FAN Yun
LI Wei
Abstract：AIM:To study the effects of phenytoin ( PHT) on the proliferation and tumor necrosis factor-α( TNF-α) expression in lipopolysaccharide ( LPS ) - treated human periodontal ligament fibroblasts ( hPDLFs ) . METHODS:hPDLFs were cultured in vitro and identified by immunocytochemistry ( ICC) SP method. The cells were cultured with 100 mg/L LPS, various concentrations of PHT and 100 mg/L LPS plus various concentrations of PHT re-spectively for 24 h. The cell proliferation was examined by MTT assay. TNF-αexpression in the cytoplasm was detec-ted by SP method. RESULTS:100 mg/L LPS inhibited the proliferation of hPDLFs and increased TNF-αexpression in the cytoplasm of hPDFLs. Phenytoin dose-dependently attenuated the effects of LPS on hPDLFs prolifereation and TNF-α expression. CONCLUSION:PHT may have protective effects on LPS-treated hPDLFs.
Keywords：phenytoinhuman periodontal ligament fibroblasts(hPDLFs)lipopolysaccharide(LPS)tumor necrosis factor-α(TNF-α)
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 396-399 )