Exploration of culture method and morphological characteristics of mouse dental follicle cells in vitro

WANG He; WU Bu-ling; DUAN Xiao-hong

doi:10.15956/j.cnki.chin.j.conserv.dent.2015.06.009

Abstract：AIM:To explore the culture method of mouse dental follicle cells ( DFCs) in vitro and to observe their morphological characteristics. METHODS:Dental germs from 3-5 days postnatal mice were digested with type I collagenase and the dental follicle tissues were dissected by enzyme isolation-tissue explant and twice-enzyme isola-tion. The morphology of the cultured DFCs was observed under inverted microscope. RESULTS:At the 4 th-5 th day in vitro, several cells were extended from part of the tissue explants and were hardly to fuse even after 2 weeks′culture. The method of enzyme isolation-tissue explant was failed because of unable to passage and amplify the cells while the method of twice-enzyme isolation got a success rate of about 85%. Scattered groups of cells were seen at the second day and they fused at the 4 th-5 th day in vitro by way of twice-enzyme isolation. Polygonal and spindle cell populations co-existed in primary culture. Almost all polygonal cells disappeared in subculture, and spindle cells became the majority of cell population. The cells of third passage grew fastest, then slowed down and tended to stop in the fourth and fifth pas-sage, respectively. After several times of passage, the cells became larger and polymorphic in shape, some dendrite-like cells were observed. CONCLUSION:The method of twice-enzyme isolation may havest considerable cells in a short time with a high success rate. Morphology of DFCs is changed with the culture time and is multiple.