Development of a novel antibacterial intrafibrillar-silicified collagen scaffold
REN Gao-tong
SUN Jia-qi
CHAI Zhi-guo
JIAO Kai
SHEN Li-juan
NIU Li-na
CHEN Ji-hua
Abstract：AIM: To develop a novel antibacterial intrafibrillar-silicified collagen scaffold. METHODS:Silicic acid solution was prepared by hydrolyzing tetraethyl orthosilicate. A chlorhexidine stabilized silicic acid was pre-pared and used as silicifying medium. Gelling time test was used to determine the minimum concentration of gluconic acid chlorhexidine that was able to stabilize silicic acid for 72 hours. The reconstituted type I collagen sponge disc was pretreated with 6. 67 × 10 -4 M poly allylamine hydrochloride for 4 h and then placed in the silicifying medium for 4 d to produce silicified collagen scaffold. Attenuated total reflection-fourier transform infrared spectroscopy ( ATR-FTIR) and transmission electron microscopy ( TEM) were used to characterize the antibacterial intrafibrillar-silicified collagen scaffold. RESULTS:2. 0% gluconic acid chlorhexidine could stabilize the silicic acid for 3 d without gelation and was used as silicifying medium. With this method, silicic acid could infiltrate into the inside space of the type I collagen and then condense into orderly deposited silicon dioxideinside collagen fibrils, resulting in intrafibrillar-silicified colla-gen scaffold doped with chlorhexidine. FTIR confirmed the incorporation of silica and chlorhexidine in the scaffold. CONCLUSION:A novel antibacterial intrafibrillar-silicified collagen scaffold is developed with reconstituted type I collagen sponge pretreated by poly allylamine hydrochloride and silicic acid stabilized by gluconic acid chlorhexidine.
Keywords：chlorhexidineantibacterial materialsintrafibrillar-silicifiedcollagen [Chinese Journal of Conservative Dentistry201525(6):363
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 363-366 )