Effect of astragalus polysaccharides on the activities of osteoblast in high glucose conditions
TENG Teng?
SUN Xin
ZHANG Ran
LIU Zhong-hao
Abstract：AIM:To evaluate the effect of Astragalus polysaccharides ( APS) on the proliferation, differentia-tion and mineralization of MC3T3-E1 cells in high glucose conditions and to study the underlying mechanisms. METH-ODS:MC3T3-E1 cells were cultured in high-glucose medium containing 0. 05(LDA), 0. 5(MDA) and 5(HDA) mg/mL of APS respectively. Cell proliferation, differentiation and mineralization were examined by MTT assay, ALP Kit and Alizarin red dye staining respectively. The expression of Runx-2, OPN and OCN were examined by RT-PCR. The cytoskeleton of the cells was observed by confocal laser scanning microscopy ( CLSM) . RESULTS: APS promoted the proliferation of MC3T3-E1 cells by LDA and MDA(P<0. 05). LDA and MDA significantly increased the ALP activity and calcified nodules (P<0. 05). Furthermore, LDA increased the gene expressions of Runx-2, OPN and OCN in a time-dependent manner (P<0. 05). CLSM observation showed that the cells in LDA group were more regular in struc-ture and had more extensions than those in the other groups at each time point. CONCLUSION: Under high glucose conditions, 0. 05 mg/mL APS promotes the proliferation, differentiation and mineralization of MC3T3-E1 cells. The effect is related to its promotion of the expression of OPN, Runx-2 and OCN.
Keywords：astragalus polysaccharideshigh glucoseosteopontinRunx-2osteocalcin
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 348-353,341 )