Dentin scaffold promotes the proliferation and differentiation of human dental pulp stem cells in vitro
YANG Xue-chao
LI Xiao-ning
WANG Wei-dong
LV Xiao-shuai
Abstract：AIM:To compare the effects of dentin surfaces treated by different endodontic irrigation on the proliferation and differentiation of human dental pulp stem cell ( hDPSCs) . METHODS:The extracted healthy human teeth were cleaned and shaped using ProTaper and K-type file rotary instrumentation. The irrigation treatments investi-gated were 52. 5 g/L sodium hypochlorite (group A), 30 mL/L hydrogen peroxide and 52. 5 g/L sodium hypochlorite (group B), 170 g/L EDTA and 52. 5 g/L sodium hypochlorite (group C). hDPSCs were seeded on the treated dentin slices with the density of 2 × 105/mL. Cell adhesion and proliferation on the dentin slices were observed by SEM and MTT method. Cell differentiation was evaluated by ALP activity assay and Real-Time PCR. RESULTS:hDPSCs ad-hered well on all dentin slices. The cells in group C showed more proliferation higher ALP level and mRNA expression of OC, DSPP and DMP-1 than those in group A and B. CONCLUSION:Dentin slices treated by EDTA-root canal preparation can promote proliferation and odontogenic differentiation of DPSCs.
Keywords：dentindental pulp stem cellstissue engineering [Chinese Journal of Conservative Dentistry201525(6):342
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 342-347 )