The role of Rac1 signal pathway in chondrogenic mechanotransduction in BMSCs
YI Fei-Zhou
ZHAO Ying
ZHAO Yin-hua
ZHANG Min
Abstract：AIM:To study the role of Racl signal pathway in chondrogenic transduction of bone marrow mes-enchymal stem cells ( BMSCs) under hydrostatic pressure. METHODS: BMSCs were isolated and cultured in vitro. The cells were then randomly divided into control group, Rac1agonist group, Rac1antagonist group, hydrostatic pres-sure group, hydrostatic pressure plus Aac1 agonist group and pressure plus Aac1 antagonist group. Cell cycle was ex-amined by flow-cytometry and cytoskeleton was observed by confocal microscopy. mRNA expression of Sox9, Aggrecan and Col-Ⅱ were detected by real-time PCR. RESULTS: Under hydrostatic pressure, S-phase fraction and the cell proliferation index of BMSCs were significantly higher than those in the control group (P≤0. 05) in a Rac1-dependent way. However, activation of the Rac1 pathway blocked F-actin cytoskeleton assembly which induced by hydrostatic pressure. The significant increase of chondrogenic marker mRNA expression in chondrogenic differentiation induced by hydrostatic pressure (P≤0. 05) was dependent on the activity of Rac1. CONCLUSION:Rac1 plays an important role in the start of the BMSCs cell cycle, cytoskeleton assembly and chondrogenic differentiation under mechanical pressure.
Keywords：hydrostatic pressurebone marrow mesenchymal stem cells ( BMSCs)Ras-related C3 botulinum toxin substrate 1 (Rac1)proliferationcell cytoskeletonchondrogenic differentiation [Chinese Journal of Conservative Dentistry201525(6):335
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 335-341 )