The effect of tamoxifen on the osteoblastic/odontoblastic differentiation of human dental pulp stem cells in vitro
WANG Juan
LIU Bao-gang
MA Feng-le
HE Xin-yao
WANG Zhi-hua
HE Wen-xi
Abstract：AIM:To investigate the effects of tamoxifen on the osteoblastic/odontoblastic differentiation of human dental pulp stem cells (hDPSCs)in vitro.METHODS:hDPSCs were treated by different concentrations of tamoxifen.Kit-8 assay was applied to assess cell proliferation.After mineralization induction culture for 2 weeks,aliz-arin red S staining was used to observe the mineral nodules,the mRNA expression of the alkaline phosphatase (ALP ),osteonectin (OCN),bone sialoprotein (BSP)and dentin sialophosphoprotein (DSPP)was detected by RT-PCR. RESULITS:Tamoxifen at 10μmol/L inhibited hDPSCs proliferation and lower concentration showed no effect.All concentration of tamoxifen increased mineral nodule formation of hDPSCs,5 μmol/L tamoxifen showed the most signifi-cant effect(P<0.05).Furthermore,the mRNA expression of ALP,OCN,BSP and DSPP was markedly upregulated by 5 μmol/L tamoxifen treatment.CONCLUSION:Tamoxifen promotes mineralization and mineralization-related genes expression and might play a vital role on the differentiation of hDPSCs.
Keywords：tamoxifenhuman dental pulp stem cellsosteoblastic differentiationodontoblastic differentiation
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 294-298 )