Comparison of the properties of the stem cells isolated from normal and inflamed dental pulps
GUAN Li-na
HE Ying
HAN Bing
SUN Xue-fei
YANG Fan
Abstract：AIM:To compare the properties of stem cells isolated from normal and inflamed dental pulps. METHODS:Dental pulp stem cells from inflamed (iDPSCs)and normal pulps (DPSCs)were cultured,purified and expanded.Cell proliferation,colony formation rate,cell surface specific markers and cell differentiation-related genes were then determined after expansion.RESULTS:CD146,CD105,CD90 and CD29 were positively expressed,but CD45 and CD31 were not expressed in both stem cells.There were no significant differences in cell surface specific mark-er expression,cell growth and colony formation rate between the two groups(P>0.05 ).RT-PCR analysis showed that the expression of RUNX2,OCN and DSPP of iDPSCs were weaker than that of DPSCs(P<0.05),but no significant difference was displayed in the expression of PPARγ(P>0.05 ).CONCLUSION:The morphology,proliferation rate and differentiation potential of iDPSCs are similar to those of DPSCs.
Keywords：inflammationdental pulp stem cellscell proliferationcell differentiation
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 288-293,307 )