Expressions of Oct4 isoforms during odontoblastic differentiation of human dental pulp stem cells
HE Ying
GUAN Li-na
SUN Xue-fei
HAN Bing
ZHANG Ya-qing
YANG Fan
Abstract：AIM:To examine the expression of Oct4 isoforms during odontoblastic differentiation of human dental pulp stem cells (hDPSCs).METHODS:hDPSCs were cultured in odontogenic induction medium .Expression of Oct4 isoforms (Oct4A and Oct4B),stem cells markers Sox2,Klf4 and DSPP were detected by RT-PCR.The pro-tein location of Oct4A in hDPSC was detected by confocal laser scanning microscope (CLSM).RESULTS:hDPSCs positively expressed CD146,CD105,CD90 and CD29,but negatively expressed CD45 and CD34.Oct4A isoform and Sox2 and Klf4 were downregulated after odontogenic induction (P<0.05),while Oct4B isoform was not detected be-fore and after induction of hDPSCs.TIP1 10 showed the same dynamic changes with Oct4A during odontoblastic differ-entiation.Moreover,Oct4A was translocated from nuclear to cytoplasm after odontogenic induction.CONCLUSION:The stemness of hDPSC are reduced during odontogenic induction.Downregulation and translocation of spliced variant Oct4A indicates that it may play a role in maintaining stemness of hDPSC.
Keywords：dental pulp stem cellOct4odontoblastic inductionalternative splicing variant
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 282-287 )