The differential proteomics of human dental follicle cells and human periodontal ligament fibroblasts analysed with Label-free technology
GONG Qing-xia
SONG Yang
LIU Jia
WANG Li-ying
JIN Zuo-lin
Abstract：AIM:To identify the differentially expressed proteins of human dental follicle cells(hDFCs)and human periodontal ligament fibroblasts(hPDLFs)with Label-free protein quantification technique.METHODS:Com-bined with ultramate 3000 nano-UPLC software,Label-free protein quantification was used to examine the total protein extraction samples from hDFCs and hPDLFs.For the proteins identified,the repeatability,the correlation,the relative content of proteins and the differentially expressed proteins of hDFCs and hPDLFs were analyzed.Bioinformatic analysis of GO and KEGG was applied for the analyses of total proteins of hDFCs and hPDLFs,as well as the differentially ex-pressed proteins.RESULTS:The relative content of proteins and the results of GO analysis of hDFCs swere similar to those of hPDLFs.22 differentially expressed proteins were identified,of which 15 were down-regulated,7 were up-regu-lated.CONCLUSION:Label-free quantitative proteomics confirmed the similar proteins profile between hDFCs and hP-DLFs.22 differentially expressed proteins provide clues for the transformation mechanism from hDFCs to hPDLFs.
Keywords：human dental follicle cells(hDFCs)human periodontal ligament fibroblasts(hPDLFs)Label-free quantitative proteomics
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 270-276 )