Function of mitochondrial respiration chain complex in bone marrow mesenchymal stem cells of SAMP6 mice
YANG Yi
HU Cheng-Hu
ZHANG Shao-Feng
JIN Yan
Abstract：AIM:To detect senescence condition,osteogenic differentiation ability and the activity of mito-chondrial respiration chain complex of bone marrow mesenchymal stem cells (BMMSCs)from senescence accelerated mouse strain prone 6 (SAMP6).METHODS:BMMSCs of SAMP6 and its control strain SAMR1 mice were isolated and cultured.The cell senescence and osteogenic differentiation ability were detected by RT-PCR,Western Blot and cell staining respectively.The function of mitochondrial respiration complex was examined by activity assay and related gene expression assay.RESULTS:Compared with control strain SAMR1 ,BMMSCs of SAMP6 BMMSCs of SAMP6 performed increase of cellular senescence ce related genes and protein level,increase ofβ-gal positive cell ratio(P<0.05),and decline of osteogenic function(P<0.05),.CONCLUSION:BMMSCs from SAMP6 perform senescence and osteogenic differentiation dysfunction with significant decline of mitochondrial respiration chain complex.
Keywords：SAMP6 micebone marrow mesenchymal stem cells(BMMSCs)mitochondrial respiration chain
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 263-269 )