Effects of mechanical pressure on the biological behavior of BMSCs cultured on titanium surfaces

SONG Yan-yan; ZHAO Ling-zhou; ZHANG Li; CAO Can; ZHANG Yu-mei

doi:10.15956/j.cnki.chin.j.conserv.dent.2015.04.006

Abstract：AIM:To investigate the effects of hydrostatic pressure on the biological behavior of bone marrow mesenchymal stem cells (BMSCs)cultured on titanium surfaces.METHODS:Pure titanium samples were divided in-to polished Ti (PT)and TiO2 nanotube array (NT)groups according to the surface modality.BMSCs were obtained from SD rats and inoculated on the titanium samples.Then the BMSCs were treated by hydrostatic pressure at 0 or 90 kPa for 1 h/d.CCK-8 method was used to determine cell proliferation at 1 ,4 and 7 d respectively after pressure treat-ment.Fluorescence staining and field-emission scanning electron microscopy (FE-SEM)were used to observe the cell skeleton and morphology after the cells were cultured for 3 d,intracellular alkaline phosphatase (ALP)activity was measured by ALP kit after the cells were cultured for 7 d.RESULTS:Anodization of pure titanium samples formed TiO2 nanotube array on the surface.Hydrostatic pressure of 90 kPa promoted the proliferation and ALP activity of BM-SCs on PT surface after the cells were cultured for 4 d (P<0.05).The actin filaments became denser and thicker.The shape of cells on all surface was more elongated and pseudopodium was also become larger after loading hydrostatic pres-sure.Hydrostatic pressure on NT surface decreased the proliferation of BMSCs after 7 d culture ,but increased F-actin and ALP expression (P<0.05 ).CONCLUSION:Mechanical pressure and substrate nanotopography may synergisti-cally affect the proliferation,cytoskeleton,morphology and intracellular ALP activity of BMSCs.