Effects of epithelial cell rests of Malassez on the osteogenesis of periodontal ligament stem cells derived from periodontitis tissue
LI Yan-jiao
LIU Wen-jia
YANG Zhen-hua
SHEN Lin
MA Xiao-jie
JIN Fang
Abstract：AIM:To study the effects of epithelial cell rests of Malassez (ERM)on the osteogenesis capaci-ty of periodontal ligament stem cells derived from periodontitis tissue (PPDLSCs).METHODS:PPDLSCs were cul-tured by low density inoculation and identified by flow cytomety.Human ERM was obtained by differential digestion and observed under phase-contrast microscope.ERMand PPDLSCs were co-cultured with 0.4 μm transwell.ALP and Runx-2 mRNA expression was examined by real-time PCR,the mineralization of the culture was evaluated by ALP staining and Alizarin Red staining.RESULTS:PPDLSCs co-cultured with ERM expressed higher mRNA levels of ALP and Runx-2.Furthermore,co-cultured PPDLSCs had stronger ALP activity and produced more mineralized nod-ules.CONCLUSION:The osteogenesis capacity of PPDLSCs can be enhanced by co-culture with ERM.
Keywords：periodontal ligament stem cells derived from periodontitis tissue (PPDSLCs)epithelial cell rests of Malassez(ERM)co-culture
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 201-205 )