Effect of mechanical injury on the expression of Notch signaling in enamel organ cells of mouse incisor
YIN Qiu-rong
TONG Juan
LUO Ying
WANG Sheng-chao
HU Yi
Abstract：AIM:To investigate the expression and role of Notch signaling in enamel organ of mouse incisor after mechanical injury.METHODS:The animal model of mechanical injury was established by grinding off 1/2 crown of mouse mandibular incisor.The animals were sacrificed at 3,5 d and 7 d respectively (n=5)after injury,the expression of Notch1 and Notch2 in cells of enamel organ was observed by immunohistochemical staining.Animals without crown grinding served as the controls(n=5 ).RESULTS:In control group,Notch1 and Notch2 were weakly expressed in stratum intermedium and stellate reticulum cells.3 days after injury,an intense expression of Notch1 and Notch2 was observed in ameloblasts and stratum intermedium cells,the expression in stellate reticulum cells was spo-radic.5 days after injury,Notch1 and Notch2 were still strongly expressed in ameloblasts,stratum intermedium and stellate reticulum cells.7 days after injury,the expression of Notch1 and Notch2 decreased in all cell layers.CON-CLUSION:Notch signaling in enamel organ cells can be activated by mechanical injury,suggesting that Notch signa-ling may be involved in the repair of incisor injury.
Keywords：notch signalingenamel organ cellsincisormechanical injury
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 193-196,237 )